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  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
Anti -Nestin Mouse mAb (ABC12155)
價(jià)格:1200.0

產(chǎn)地:ABC

貨號(hào):ABC12155

規(guī)格:100UL

備注:

  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
  • Anti -Nestin Mouse mAb (ABC12155)
產(chǎn)品信息
產(chǎn)品名稱(chēng) 產(chǎn)品編號(hào) 規(guī)格
Anti -Nestin Mouse mAb ABC12155 100UL
Product Information
Protein full name Nestin
Synonyms NES, Nbla00170, nestin
Immunogen Recombinant protein corresponding to Mouse Nestin
Uniprot ID Q6P5H2
Isotype IgG2b,κ
Purity Affinity purification
Subcellular location Intermediate filament
Applications
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 kidney
背景

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

圖像
IHC analysis of Nestin (GB12137).
Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of Nestin (GB12137).
Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of Nestin (GB12137).
Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IF analysis of Nestin (GB12137).
Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
IF analysis of Nestin (GB12137).
Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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